Cspb promoter
Webunder the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. Immu-nostaining microscopy and flow cytometric analysis revealed that PorB-AmyA, PorC-AmyA, and PorH-AmyA were displayed on the C. glutamicum cell surface. AmyA activity was only detected in the cell fraction of C. WebJul 5, 2016 · A total of 84 predicted promoter sequences located upstream of different classes of proteins including heat shock proteins, cell-envelope proteins, and proteins resistant against toxic metals (based on similarity) …
Cspb promoter
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WebJan 1, 2008 · The promoter and signal sequence of the cspB gene encoding PS2, the major protein secreted by the organism, have been used in many studies of heterologous protein secretion in C. glutamicum [43,44 ... WebApr 18, 2024 · Construction of gadB2 expression strains under different RBS sequence and promoter. The nucleotide sequences of all primers are listed in Table 2.A series of RBS sequence derived from the consensus sequence AGGAG and an AS of 6–8 nt was designed and applied for expressing gadB2.For expressing with normal RBS sequence and AS of …
WebSep 22, 2007 · Corynebacterium glutamicum is an important microorganism in the industrial production of amino acids. We engineered a strain of C. glutamicum that secretes α … WebThe xfp gene encoding PKT was cloned from Bifidobacterium animalis and overexpressed under the strong cspB promoter in C. glutamicum. A functional enzyme was detected in an L-Glu-producing strain of C. glutamicum (odhA). When cells of this producer strain with the xfp gene and those without the xfp gene were cultivated in a controlled ...
WebDownload scientific diagram Genetic features of the cspB promoter region of C. glutamicum ATCC 14067 and visualization by SDS-PAGE of proteins binding in front of the cspB gene. (a) The ... WebJan 1, 2000 · The cspB promoter (PcspB) described by Peyret et al. (1993) was isolated as a. 554 bp Eco RI \ Eco NI fragment and ligated to an Eco NI \ Bam HI adaptor constructed with the two synthetic oligo-
WebThe major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides ... a cis-element found in the promoter region of mammalian major histocompatability complex class II genes, which has the consensus sequence CTGATTGGC/TC/TAA [4,5]. The underlined penta-
WebDec 1, 2011 · DNA fragments containing the promoter sequence of CspB from C. glutamicum and the signal sequence of CspA from C. ammoniagenes were fused to the CspB50TEV-Teriparatide gene. This synthesized construct was then inserted into the pPK4 plasmid and incorporated into the YDK010 bacterial strain, which is a derivative of wild … share the spirit festival wominjekaWebJan 16, 2024 · The Duffer Brothers, Brittany Howard, Lyle Lovett, Qui Nguyen, Angie Thomas, Billy Bob Thornton, Mary Steenburgen and Jesmyn Ward Among Featured … poplar road surgeryWebJul 3, 2024 · The promoter of elongation factor Tu (tuf), which is known as a strong promoter in C. glutamicum, was amplified by PCR using primers P15 and P16 for the … poplar rowing clubWebDec 31, 2002 · AmyA expression under the control of cspB promoter was assumed to be induced when raw starch was used as the sole carbon source. These results indicate that efficient simultaneous saccharification and fermentation of raw corn starch to L-lysine were achieved by C. glutamicum secreting AmyA using the cspB promoter and signal … poplar ridge winery nyWebSep 4, 2003 · Synthesis of CspB is high when these strains are grown on lactate, acetate, gluconate and tartrate but low when the carbon sources are glucose, fructose, sucrose … share the spirit cbs wikiWebMay 9, 2009 · We used α-amylase from Streptococcus bovis 148 (AmyA) as a model protein to be displayed on the C. glutamicum cell surface. AmyA was fused to the C terminus of the porins PorB, PorC, or PorH. Expression vectors using fused proteins under the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. share the spirit redwood falls mnWebCSPB may refer to the following: California State Personnel Board, one of California's state constitutional offices; Credit Suisse Private Banking, a financial services company in … sharethesub