WebBioactive Small Molecules D3922 Print Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza- s -Indacene) … WebJan 9, 2024 · The BODIPY 493/503 (D3922; Thermo Fisher Scientific, USA) staining was performed for primary IECs, as described by Wei et al. . The laser scanning confocal microscope (Leica Microsystems, Germany) …
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WebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific) in PBS for 1 hr, and then fixed in 4% paraformaldehyde (PFA) in PBS for 10 min at room temperature. WebNov 18, 2024 · DFCP1 is an ER-associated protein with two PI (3)P-binding FYVE domains. In WT cells, myc-DFCP1 formed very small punctate structures ( Figure 1E) ( Axe et al., 2008; Li et al., 2024 ). In SKO cells, the myc-DFCP1 structures appeared highly irregular and significantly enlarged ( Figure 1, E and F).
WebFeb 9, 2024 · To visualize the accumulation of lipids in response to OA treatment, we used boron-dipyrromethene (BODIPY) 493/503 (D3922, Thermo Fisher Scientific, MA, USA), which labels specifically ... WebFeb 8, 2013 · This Thermo Scientific full-size Ultrapure mixed bed ion exchange hose nipple cartridge is an economical way to purify water volumes up to 75L/hour. It is constructed of 100% virgin polypropylene cartridge with superior quality resin. The color change indicator identifies when resin is exhausted. It is designed for non-pressurized …
WebJan 16, 2024 · Briefly, 2D or 3D 3T3-L1 cells were incubated in a mixture of 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor 594 phalloidin (# 20553, Funakoshi, Tokyo, Japan), and DAPI (# D523, Dojindo, Kumamoto, Japan), at 1:1000 dilutions in PBS for 1 hr, and thereafter fixed in 4% paraformaldehyde in PBS for … WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …
WebJul 4, 2024 · They were cultured in Dulbecco’s Modified Eagle’s medium (Thermo Fisher Scientific) media containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) in an incubator at 28℃. ... (D3922; Thermo Fisher Scientific) for 30 min, followed by thrice PBS washes. The IECs were observed using a laser scanning confocal microscope …
WebAug 1, 2024 · For BODIPY lipid staining [6–8], 4% paraformaldehyde fixed 3D sphenoids were incubated in 0.1% BODIPY (# D3922, Thermo Fisher Scientific), 0.1% DAPI (#D523, Doujin, Japan) and 0.1% phalloidin (#20,553, Funakoshi, Japan) in phosphate buffered saline containing 3% bovine serum albumin for 3 hrs. how to search dates in facebook messengerWebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 … how to search dba in michiganWebSep 1, 2024 · Briefly, HepG2 cells were fixed with 4% paraformaldehyde for 10 min, then washed three times with phosphate buffer saline (PBS). After washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific, MA, USA) at room temperature for 1 h, followed by three washes with PBS. how to search death records for freeWebMeasurement Range: -50 to +392°F/-45 to +200°C NSF® Certified One-button operation 3 mm tip 5"/12.7 cm stem Shatterproof Food-safe ABS plastic with BioCote® Stainless … how to search dealer inventoryWebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, … how to search dates in excelWebFeature papers are submitted upon individual invitation or recommendation by the scientific editors and must receive positive feedback from the reviewers. ... Alternatively, for BODIPY staining, 3D spheroids in 6 super-low-attachment well dishes were incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 1 h ... how to search define in verdiWebFeb 24, 2024 · Finally, BODIPY493/503 (#D3922, Thermo Fisher Scientific, USA) staining was performed to check the lipid droplet formation. ... USA) according to the manufacturer’s instruction. SYBR Green qPCR Master Mix (#K0251, Thermo Fisher Scientific, USA) was used for quantitative real-time PCR amplification with a CFX96 real-time PCR detection … how to search deleted accounts roblox