WebIPTG配制 . Prepare a 1 M solution of IPTG (Isopropyl-β-D-thiogalactoside; Isopropyl-β-D-thiogalactopyranoside) by dissolving 2.38 g of IPTG in dd water and adjust the final volume to 10 ml. Filter sterilize before use. 氯霉素配制 . Chloramphenicol 34 mg/ml in ethanol. Store at -20℃. Use at 34 µg/ml. 注意事项 WebIPTG配制 . Prepare a 1 M solution of IPTG (Isopropyl-β-D-thiogalactoside; Isopropyl-β-D-thiogalactopyranoside) by dissolving 2.38 g of IPTG in dd water and adjust the final …
IPTG 异丙基-β-D-硫代半乳糖苷 - 上海懋康生物科技有限公司
WebIPTG (isopropylthio-β-galactoside) is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures. … WebIPTG即Isopropyl β-D-1-thiogalactopyranoside,也称Isopropyl β-D-thiogalactoside,中文名为异丙基-β-D-硫代半乳糖苷。 分子式为C9H18O5S ,分子量为238.30,CAS Number … phone to printer android app
IPTG (Isopropyl-β-D-thiogalactopyranoside) VWR
WebIsopropyl β-D-1 thiogalactopyranoside (IPTG) Luria Broth (LB) powder or Tryptone, Yeast extract and NaCl Agar Sodium chloride (NaCl) Sodium phosphate dibasic (Na 2 HPO 4) Sodium Dodecyl Sulfate (SDS) Dithiothreitol (DTT) Glycerol Bromophenol Blue Glycine Tris base Coomassie protein gel stain 3.1 Solutions and buffers Step 1. Reagents Antibiotic WebJan 16, 2024 · Abstract This work assesses the effect of chemical induction with isopropyl β-D-1-thiogalactopyranoside (IPTG) on the expression of enhanced green fluorescent protein (eGFP) by planktonic and biofilm cells of Escherichia coli JM109 (DE3) transformed with a plasmid containing a T7 promoter. WebIsopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high ... how do you spell honey badger