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WebTaken on May 1, 2016 . All rights reserved . About; Jobs; Blog; Developers; Guidelines; Privacy; Terms; Help; Report abuse; Help forum; English WebOR3R, 5’-ACGACATGCTTATGCCTCTC-3’, PCR amplification was performed with the fol-lowing conditions: a denaturing step at 95 °C for 5 min, followed by 35 cycles (95 °C for 30 s, 55 °C for 30 s, 72 °C for 30 s), and a final step of 7 min a t 72 °C. The 200-bp PCR product was purified and ligated into a pUC 19-T vector (Takara, China). WebOR3R, 5'-ACGACATGCTTATGCCTCTC-3', PCR amplification was performed with the following conditions: a denaturing step at 95 °C for 5 min, followed by 35 cycles (95 °C for 30 s, 55 °C for 30 s, 72 °C for 30 s), and a final step of 7 min at 72 °C. The 200-bp PCR product was purified and ligated into a pUC 19-T vector (Takara, China). trust issues in the bible