Phospho fak antibody
WebPhospho-FAK (Tyr576/577) Antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 576/577. This antibody may cross-react with other activated … Web# V9131) and anti-tyrosin 397-phospho-focal adhesion kinase (FAK) (rabbit polyclonal, Fisher Scientific, Cat. # 700255) specific primary antibodies diluted 1:300 and 1:500, respectively, in blocking solution at room temperature for 1 hour by placing samples upside-down on a drop (50 µL) of antibody solution. After washing in 1xPBS cells were ...
Phospho fak antibody
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WebApr 4, 2024 · Electrical stimulation for 20 h stained with anti-phospho-FAK (pY397) antibody (C) or electrical stimulation for 20 h stained with anti-phospho-c-Src (pY418) antibody (F). Bars: 20 μm. Figure 6. Changes in phosphorylated proteins, tyrosine-phosphorylated FAK (A), and tyrosine-phosphorylated c-Src (B) were examined by ELISA. WebWestern blot - Anti-FAK (phospho S910) antibody (ab4794) Specificity for detecting FAK pS910 phosphorylation using a phospho-specific antibody (PSSA): Cell extracts prepared …
WebIntriguingly, Sema6C-binding antibodies have been recently shown to stimulate pancreatic carcinoma cell proliferation, possibly neutralizing a suppressor activity ... B Western blotting analysis of phospho-FAK, total FAK, and YAP levels in detached Sema6C-overexpressing and control BxPC-3 cells in suspension; GAPDH provided a protein loading ... WebPhospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 397. RRID: AB_2834829 Cite Format: Affinity Biosciences Cat# AF3398, RRID:AB_2834829. Conjugate: Unconjugated. Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho …
WebHuman Phospho-FAK (Y397) Antibody Summary Species Reactivity Human Specificity Detects human FAK when phosphorylated at Y397. Source Polyclonal Rabbit IgG Purification Antigen and protein A Affinity-purified Immunogen Phosphopeptide containing human FAK Y397 site Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. WebPVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-FAK (Y925) Monoclonal Antibody (Catalog # MAB44671) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under …
WebWestern blotting for human FAK (pY397). Human endothelial cells were treated with 1 mM pervanadate, a general inhibitor of protein tyrosine phosphotases, for 15 minutes at 37°C then either left untreated (lane 1) or …
WebNov 7, 2005 · Rabbit polyclonal caspase-3, -9, FAK, phospho-FAK Y576/577, and phospho-paxillin Y118 antibodies were purchased from Cell Signaling Technology, and antibodies against phospho-FAK Y397 were obtained from Calbiochem. HRP-conjugated anti–mouse and anti–rabbit secondary antibodies were purchased from GE Healthcare. bing rewards sign in page nWebPhospho-FAK (Ser910) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. 4 … da-100 analyzing data with power biWebHuman Phospho-FAK (S843) Antibody Summary Species Reactivity Human Specificity Detects human FAK when phosphorylated at S843 in direct ELISAs and Western blots. Source Monoclonal Mouse IgG 1 Clone # 743101 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Phosphopeptide containing the human FAK … da0zq1mb8f0 rev f schematicWebHuman Phospho-FAK (Y397) Antibody Summary. Detects human FAK when phosphorylated at Y397. Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack … bing rewards sucheWeb公司IL-9 elisa酶联免疫试剂盒图片质量可靠,价格便宜,免费代测。周期短,数据准确。种属:人、大小鼠、豚鼠、兔子、... da-100 certification downloadWebPolyclonal Antibody for studying FAK (Tyr925) phosphate. Cited in 135 publications. Validated for Western Blotting, Immunoprecipitation. Available in 2 sizes. Highly specific … bing rewards status level requirementWebAug 2, 2024 · Rabbit antibodies against human MMP-2, MMP-9, urokinase, PI3K, and phosphorylated-PI3K were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA). 4.2. Cell Culture and MTT Assay. ... (FAK), phosphorylated FAK (phospho-FAK), phosphoinositide 3-kinase (PI3K), phospho-PI3K, AKT, phospho-AKT, mTOR and phospho … da 100 certification exam fees