Phospho ck2 antibody
WebPhospho-CK2 Substrate [ (pS/pT)DXE] MultiMab™ Rabbit mAb mix recognizes endogenous proteins containing a pS/pTDXE motif, which is a CK2 phosphorylation consensus … WebListed below are anti-CK2 antibodies from multiple suppliers. CK2 is a reported alias name for the human gene CSNK2B, or 'casein kinase 2 beta'. The 215-amino acid protein is a member of the Casein kinase 2 subunit beta family. See the supplier page to learn important antibody details, such as target specificity and designated applications.
Phospho ck2 antibody
Did you know?
WebType Antibody Immunogen Synthetic phosphopeptide corresponding to residues surrounding pSer19 of human Chk2 Conjugate Unconjugated Form Liquid Purification Antigen affinity chromatography Storage Buffer 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol Contains no preservative Storage Conditions -20°C Species … WebThe antibody was specific for phosphorylated Cdc37 and did not crossreact with other CK2 substrates such as Hsp90 and FK506-binding protein 52. Using this antibody, we showed that complexes of Hsp90 with its client signaling kinases, Cdk4, MOK, v-Src, and Raf1, contained the CK2-phosphorylated form of Cdc37 in vivo.
WebDec 26, 2024 · The CCA cell lines all showed a reduction in multiple phospho-CK2 substrates with apparent molecular masses of approximately 200, 180, 100, 60, 37, 35, and 33 kDa as indicated by arrows in the Figure ... We measured CK2 activity indirectly by using an antibody against the phospho-CK2 substrate (motif pS/pTDXE) to measure the amount of ... WebDec 22, 2024 · CK2 kinase activity is often determined by measuring the phosphorylation of one of the down-stream signaling molecules, namely Akt, which is phosphorylated by CK2 at serine129 . Phosphorylation of Akt is detected by a phospho-specific antibody directed against the CK2 phosphorylation site serine 129.
WebApr 6, 2024 · Inhibition of CK2 decreased PD-L1 protein levels by promoting its degradation and resulted in the release of CD80 from DC to reactivate T-cell function. In a syngeneic mouse model, combined... WebAug 23, 2024 · The Phospho-CK2 Substrate [ (pS/pT)DXE] MultiMab ™ Rabbit mAb mix Antibody has been validated for the following applications: Western Blot. Phospho-CK2 …
WebOct 8, 2007 · Here we describe a new phospho-specific antibody against Cdc37 that recognizes recombinant purified Cdc37 only when incubated with CK2 in the presence of Mg 2+ and ATP. The replacement of Ser13 in Cdc37 by nonphosphorylatable amino acids abolished binding to this antibody.
WebCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha′ chains contain the catalytic site. … csl iat sensorWebPhospho-LCK (Tyr192) Antibody (44-844G) in WB. Peptide Competition. Background extracts alone (1) or with added recombinant active human Lck protein (2-5) were … eagle river wi christmasWebPhospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also cross-reacts with proteins and peptides that only harbor an RXXpS/pT motif. Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb rec-ognizes phospho-serine in a KS*P motif. The ... eagle river wi commercial real estateWebApr 11, 2024 · A CK2 phospho-substrate antibody mix can be used as an indicator of CK2 activity. IKras PDAC cells grown in the presence of Dox were treated overnight with the … eagle river wi craigslistWebInvitrogen Anti-Phospho-CK2 alpha-1 (Tyr255) Polyclonal, Catalog # PA5-40226. Tested in Western Blot (WB), Immunohistochemistry (IHC) and ELISA (ELISA) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (1 … eagle river wi commercial business for saleWebRabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1/ABL2. Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y393/Y429 of human ABL1/ABL2. (PAB12190) - Products - Abnova cslhwdeviceopsWebNov 1, 2007 · A new phospho‐specific antibody against Cdc37 is described that recognizes recombinant purified CDC37 only when incubated with CK2 in the presence of Mg2+ and ATP and showed that complexes of Hsp90 with its client signaling kinases, Cdk4, MOK, v‐Src, and Raf1, contained the CK2‐phosphorylated form of C dc37 in vivo. The … csl iberia